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Overview

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  Immunofluorescence analysis of Hela cell. 1,NF-YA Polyclonal Antibody(red) was diluted at 1:200(4° overnight). LC3A mouse Monoclonal Antibody(5G10)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted a
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  Western Blot analysis of various cells using NF-YA Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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  Western blot analysis of lysates from HepG2, 293, and COLO205 cells, using NFYA Antibody. The lane on the right is blocked with the synthesized peptide.
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  Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).