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產(chǎn)品中心

GAK rabbit pAb

一鍵復(fù)制產(chǎn)品信息

ES2393

規(guī)格：	價(jià)格：
50μL	￥1280.00
100μL	￥1980.00

下載說明 ①

Overview

Product name:	GAK rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	GAK; Cyclin-G-associated kinase
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human GAK. AA range:101-150
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	144kD
GeneID:	2580
Human Swiss-Prot No:	O14976
Cellular localization:	Cytoplasm, perinuclear region . Golgi apparatus, trans-Golgi network . Cell junction, focal adhesion . Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
Background:	cyclin G associated kinase(GAK) Homo sapiens In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015],

Western Blot analysis of various cells using GAK Polyclonal Antibody
Western blot analysis of lysates from 293 cells, using GAK Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HepG2 cells using GAK antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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