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Overview

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  Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
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  Immunofluorescence analysis of Hela cell. 1,Claudin-1 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
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  Western Blot analysis of various cells using Claudin-1 Polyclonal Antibody diluted at 1:1000
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  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Claudin 1 Antibody. The picture on the right is blocked with the synthesized peptide.