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ATP5H rabbit pAb

一鍵復(fù)制產(chǎn)品信息

ES1728

規(guī)格：	價(jià)格：
50μL	￥1280.00
100μL	￥1980.00

下載說(shuō)明 ①

Overview

Product name:	ATP5H rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	ATP5H; My032; ATP synthase subunit d; mitochondrial; ATPase subunit d
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human ATP5H. AA range:111-160
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	26kD
GeneID:	10476
Human Swiss-Prot No:	O75947
Cellular localization:	Mitochondrion. Mitochondrion inner membrane.
Background:	Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010],

Western Blot analysis of various cells using ATP5H Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from HepG2 and Jurkat cells, using ATP5H Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HepG2 cells using ATP5H antibody.
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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