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One Step SYBR Green RT-PCR Mix
一鍵復(fù)制產(chǎn)品信息
EQ007
規(guī)格： 價格：
20μl x 100rxn ￥980.00
20μl x 500rxn ￥3800.00

Overview 文獻(xiàn)(8)

Product name: One Step SYBR Green RT-PCR Mix
產(chǎn)品介紹: This product is a special reagent for Real Time One Step RT-PCR using the probe method. Using this product for Real Time RT-PCR reaction can continuously perform reverse transcription and PCR amplification in the same reaction tube. It is simple to operate and can effectively prevent contamination. Since this reaction system can monitor the amplified products in real time, the detection sensitivity is greatly improved, and the electrophoresis step after PCR reaction is omitted, which is very suitable for the detection of RNA viruses.This product uses high-efficiency reverse transcriptase and high-specificity hot-start Taq DNA polymerase to perform stable and efficient Real Time One Step RT-PCR reactions. For the fluorescent quantitative PCR instrument that uses ROX as the calibration dye, this product is equipped with a separate ROX dye to correct the fluorescent signal error generated between the wells of the quantitative PCR instrument.
產(chǎn)品特點(diǎn): 1.只需加入RNA，即可在同一PCR管內(nèi)連續(xù)進(jìn)行逆轉(zhuǎn)錄和PCR擴(kuò)增，操作非常簡便。

2.對擴(kuò)增產(chǎn)物進(jìn)行實(shí)時檢測，大大提高了檢測靈敏度，并能有效防止污染。

3.優(yōu)選的三代逆轉(zhuǎn)錄酶配套熱啟動Taq酶使用以獲得最佳的擴(kuò)增效果，有效降低了非特異性擴(kuò)增。

4.省略了PCR反應(yīng)后的電泳步驟，非常適合于微量RNA的檢測。
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