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人胎盤(pán)鈣黏蛋白(P-Cadherin)微量上樣酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK9588MS

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview

Product name:	Human P-Cadherin(Cadherin, Placental) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	CDH3; CDHP; HJMD; PCAD; Cadherin 3; type 1
Assay Type:	Sandwich
Sensitivity:	36 pg/mL
Standard:	4000 pg/mL
Detection Range:	62.50-4000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal Transduction;Neuroscience
Uniprot ID:	P22223
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human P-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human P-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human P-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human P-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
4000.00	1.934	1.838
2000.00	1.571	1.475
1000.00	1.178	1.082
500.00	0.830	0.734
250.00	0.516	0.420
125.00	0.371	0.275
62.50	0.251	0.155
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant P-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of P-Cadherin in samples.

Matrix	Recovery range	Average
serum(n=5)	83-113%	98%
EDTA plasma(n=5)	85-98%	92%
Heparin plasma(n=5)	95-105%	100%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.