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大鼠氨基端前腦鈉素(NT-ProBNP)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK8211

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說明 ①

Overview 文獻(xiàn)(3)

Product name:	Rat NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Reactivity:	Rat
Alternative Names:	NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates,cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Cardiovascular Diabetology

Dapagliflozin reduces the vulnerability of rats with pulmonary arterial hypertension-induced right heart failure to ventricular arrhythmia by restoring calcium handling

日期：2022-09-28 IF：8.949
Bioengineered

Chronic Sigma 1 receptor activation alleviates right ventricular dysfunction secondary to pulmonary arterial hypertension

日期：2022-04-27 IF：3.269
Bioengineered

Chronic Sigma 1 receptor activation alleviates right ventricular dysfunction secondary to pulmonary arterial hypertension

日期：2023-09-05 IF：3.2

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.252	2.170
5.00	1.703	1.621
2.50	0.875	0.793
1.25	0.739	0.657
0.63	0.521	0.439
0.32	0.357	0.275
0.16	0.226	0.144
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.

Matrix	Recovery range	Average
serum(n=5)	83-97%	90%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	95-108%	101%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.