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大鼠3-羥基3-甲基戊二酰輔酶A還原酶(HMGCR)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK6333

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說明 ①

Overview 文獻(xiàn)(1)

Product name:	Rat HMGCR(3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase) ELISA Kit
Reactivity:	Rat
Alternative Names:	HMG-CoA Reductase; Hydroxymethylglutaryl-CoA Reductase
Assay Type:	Sandwich
Sensitivity:	0.131 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;
Uniprot ID:	P51639
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGCR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGCR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGCR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGCR in the samples is then determined by comparing the OD of the samples to the standard curve.

Pharmacological Reports

Changes in regulators of lipid metabolism in the brain: a study of animal models of depression and hypothyroidism

日期：2022-08-11 IF：3.919

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.136	2.048
10.00	1.625	1.537
5.00	1.142	1.054
2.50	0.835	0.747
1.25	0.563	0.475
0.63	0.315	0.227
0.32	0.168	0.080
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HMGCR and the recovery rates were calculated by comparing the measured value to the expected amount of HMGCR in samples.

Matrix	Recovery range	Average
serum(n=5)	88-102%	95%
EDTA plasma(n=5)	80-92%	86%
Heparin plasma(n=5)	90-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMGCR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.