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人內(nèi)皮素2(EDN2)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK5069

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human EDN2(Endothelin 2) ELISA Kit
Reactivity:	Human
Alternative Names:	ET2; PPET2; Preproendothelin-2
Assay Type:	Competitive Inhibition
Sensitivity:	4.87 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	Serum, plasma and other biological fluids.
Assay length:	2h
Research Area:	Cardiovascular biology;
Uniprot ID:	P20800
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human EDN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EDN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EDN2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	0.199
500.00	0.396
250.00	0.557
125.00	0.857
62.50	1.197
31.25	1.511
15.63	1.793
0.00	2.123

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant EDN2 and the recovery rates were calculated by comparing the measured value to the expected amount of EDN2 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-103%	96%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	81-95%	99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EDN2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.