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大鼠胱抑素C(Cys-C)酶聯(lián)免疫吸附檢測試劑盒

一鍵復制產(chǎn)品信息

ELK11021

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview 文獻(7)

Product name:	Rat Cys-C(Cystatin C) ELISA Kit
Reactivity:	Rat
Alternative Names:	CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
Assay Type:	Sandwich
Sensitivity:	0.51 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Stem Cells;Cardiovascular;Cell Biology;Tags & Cell Markers;
Uniprot ID:	P14841
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

PHYTOMEDICINE

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日期：2023-08-06 IF：5.6
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY

Reno-protective effect of nicorandil and pentoxifylline against potassium dichromate-induced acute renal injury via modulation p38MAPK/Nrf2/HO-1 and Notch1/TLR4/NF-κB signaling pathways

日期：2024-09-05 IF：3.6
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Early diagnostic biomarkers for acute kidney injury using cisplatin-induced nephrotoxicity in rat model

日期：2023-10-06 IF：3.3
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Reno-protective effect of fenofibrate and febuxostat against vancomycin-induced acute renal injury in rats: Targeting PPARγ/NF-κB/COX-II and AMPK/Nrf2/HO-1 signaling pathways

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Immunopharmacology and Immunotoxicology

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日期：2025-03-17 IF：2.9

標準曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	2.096	2.009
50.00	1.686	1.599
25.00	1.120	1.033
12.50	0.828	0.741
6.25	0.567	0.480
3.13	0.318	0.231
1.57	0.248	0.161
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.

Matrix	Recovery range	Average
serum(n=5)	88-102%	95%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	79-93%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.