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產(chǎn)品中心

雌二醇(E2)酶聯(lián)免疫吸附檢測試劑盒

一鍵復制產(chǎn)品信息

ELK10219

規(guī)格：	價格：
48T	￥1680.00
96T	￥2400.00
96T*5	￥10200.00

下載說明 ①

Overview 文獻(9)

Product name:	E2(Estradiol) ELISA Kit
Reactivity:	General
Alternative Names:	17B-Estradiol; Oestradiol; Beta-Estradiol
Assay Type:	Competitive Inhibition
Sensitivity:	14.45 pg/mL
Standard:	3000 pg/mL
Detection Range:	46.88-3000 pg/mL
Sample Type:	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Reproduction Open

Influence of ovarian stromal cells on human ovarian follicle growth in a 3D environment

日期：2023-12-21 IF：8.3
Stem Cell Research & Therapy

Model construction and drug therapy of primary ovarian insufficiency by ultrasound-guided injection

日期：2024-02-20 IF：7.5
Phytomedicine

Dihydrotanshinone I targets ESR1 to induce DNA double-strand breaks and proliferation inhibition in hepatocellular carcinoma

日期：2024-07-25 IF：6.7
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Leydig Cells in Immunocastrated Polish Landrace Pig Testis: Differentiation Status and Steroid Enzyme Expression Status

日期：2024-06-10 IF：6.3
Journal of Ethnopharmacology

Jianpi Antai formula prevents miscarriage by repressing M1 polarization of decidual macrophages through ubiquitination of NLRP3 mediated by MARCH7

日期：2024-01-18 IF：5.4
Journal of Traditional and Complementary Medicine

Effect and mechanism of pearl on ovarian function of rats with premature ovarian failure induced by tripterygium glycosides

日期：2023-03-01 IF：4.221
Scientific Reports

Impact of light spectrum electromagnetic radiation variations on performance and hormonal profiles in laying hens

日期：2022-03-12 IF：3.8
Theriogenology

Senescence and autophagy relation with the expressional status of non-canonical estrogen receptors in testes and adrenals of roe deer (Capreolus capreolus) during the pre-rut period

日期：2022-03-29 IF：3
General and Comparative Endocrinology

The role of the opioid peptide dynorphin during the seasonal and gonadotropin-induced ovarian recrudescence in the gecko

日期：2025-03-17 IF：2.1

標準曲線

Concentration (pg/mL)	OD	Corrected OD
3000.00	0.189
1500.00	0.397
750.00	0.596
375.00	0.833
187.50	1.197
93.75	1.592
46.88	1.789
0.00	2.123

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	88-102%	95%
Heparin plasma(n=5)	79-97%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.