国产a级毛片_日韩精品一区二区三区在线观看_欧美色图片区_亚洲性无码av中文字幕 ,亚洲国产精久久久久久久_欧美久久综合网_亚洲欧美视频一区二区_秋霞午夜一级理论片久久 ,久久人人澡人人爽人人爱_亚洲成av人在线观看影院_2020亚洲欧美国产日韩

產(chǎn)品中心

一步法小鼠新喋呤(NPT)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK100ES

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview

Product name:	EasyStep Mouse NPT(Neopterin) ELISA Kit
Reactivity:	Mouse
Alternative Names:	Neopterin
Assay Type:	Competitive Inhibition
Sensitivity:	0.4 nmol/L
Standard:	30 nmol/L
Detection Range:	0.469-30 nmol/L
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Cytokine;Infection immunity;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NPT antigen, and the Mouse NPT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse NPT to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse NPT in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (nmol/L)	OD	Corrected OD
30.00	0.215	0.000
15.00	0.352	0.000
7.50	0.567	0.000
3.75	0.833	0.000
1.88	1.157	0.000
0.94	1.585	0.000
0.47	1.715	0.000
0.00	2.226	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NPT and the recovery rates were calculated by comparing the measured value to the expected amount of NPT in samples.

Matrix	Recovery range	Average
serum(n=5)	84-98%	91%
EDTA plasma(n=5)	80-96%	88%
Heparin plasma(n=5)	82-98%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NPT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.