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小鼠磷酸化胰島素受體β亞單位IR (Phospho) [pY1162/1163]酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK0766

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說明 ①

Overview 文獻(1)

Product name:	Mouse phospho-Insulin Receptor, β Subunit(pTyr1162/1163) ELISA Kit
Reactivity:	Mouse
Alternative Names:	p-IR
Assay Type:	Sandwich
Sensitivity:	0.68 U/mL
Standard:	100 U/mL
Detection Range:	1.57-100 U/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;Endocrinology;Autoimmunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IR (Phospho) [pY1162/1163]. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IR (Phospho) [pY1162/1163]. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IR (Phospho) [pY1162/1163], biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IR (Phospho) [pY1162/1163] in the samples is then determined by comparing the OD of the samples to the standard curve.

Neurobiology of Disease

Prenatal stress aggravates age-dependent cognitive decline, insulin signaling dysfunction, and the pro-inflammatory response in the APPNL-F/NL-F mouse model of Alzheimer's disease

日期：2023-06-29 IF：6.1

標準曲線

Concentration (U/mL)	OD	Corrected OD
100.00	2.079	1.978
50.00	1.593	1.492
25.00	1.183	1.082
12.50	0.857	0.756
6.25	0.509	0.408
3.13	0.325	0.224
1.57	0.199	0.098
0.00	0.101	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IR (Phospho) [pY1162/1163] and the recovery rates were calculated by comparing the measured value to the expected amount of IR (Phospho) [pY1162/1163] in samples.

Matrix	Recovery range	Average
serum(n=5)	82-98%	90%
EDTA plasma(n=5)	82-94%	88%
Heparin plasma(n=5)	80-97%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IR (Phospho) [pY1162/1163] and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.